What is Phosphate Buffered Saline (PBS) used for?

Phosphate Buffered Saline (PBS) is primarily used in biological research as a buffer solution for dissolving biological samples. Its formulation helps maintain a stable pH, making it ideal for applications like cell culture, immunoassays, and molecular biology experiments. The sterile nature of PBS prevents contamination, ensuring accurate results. For example, in cell culture, PBS is frequently used to rinse cells before trypsinization, as it helps maintain osmotic balance and cell viability.

Is PBS 1X suitable for use in cell culture?

Yes, PBS 1X is suitable for cell culture applications. It provides a balanced salt solution that supports the physiological conditions necessary for cell survival and growth. With its sterile formulation, PBS helps in processes such as washing cells, preparing samples for flow cytometry, and diluting other reagents. Many researchers use PBS to remove serum before enzymatic detachment of adherent cells, ensuring minimal disruption to experimental results.

How should Phosphate Buffered Saline be stored?

Phosphate Buffered Saline should be stored at room temperature in a cool, dry place, away from direct sunlight. It is recommended to keep PBS tightly sealed in its original container to prevent contamination. While PBS can be stored for extended periods, it is advisable to check for any visible contaminants before use. Proper storage ensures the buffer retains its efficacy, which is critical for sensitive applications such as immunofluorescence staining.

Can PBS be used in molecular biology techniques?

Yes, PBS is widely used in various molecular biology techniques, including PCR (Polymerase Chain Reaction), Western blotting, and ELISA (Enzyme-linked Immunosorbent Assay). Its role as a buffering agent aids in maintaining a stable pH during reactions, which is crucial for enzyme activity and specificity. For instance, during the dilution of antibodies or nucleic acids, PBS helps maintain the integrity of the reagents, contributing to reproducible experimental outcomes.

Is PBS 1X compatible with mammalian cells?

PBS 1X is compatible with mammalian cells, as it mimics physiological conditions, making it an ideal medium for cell handling and experiments. Its isotonic nature prevents osmotic shock, ensuring cell health during procedures like trypsinization or washing. Researchers often utilize PBS when preparing mammalian cells for flow cytometry assays, as it aids in maintaining cell viability while optimizing staining protocols.

What are the components of Phosphate Buffered Saline?

Phosphate Buffered Saline typically contains sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate. These components work together to create an isotonic and buffered environment, maintaining a stable pH around 7.4. This balanced formulation is crucial for biological applications, as it prevents changes in cellular environments that could disrupt normal function. Understanding these components can help researchers select the right medium for their specific experiments.

Can I use PBS for preparing samples for flow cytometry?

Absolutely, PBS is a preferred buffer for preparing samples for flow cytometry. Its isotonic properties help preserve cell morphology and viability, which are essential for accurate analysis. By using PBS to dilute samples, researchers can effectively minimize background noise and improve the clarity of cytometric data. Additionally, many protocols recommend washing cells with PBS to reduce nonspecific binding of antibodies, enhancing the quality of results.

Is the PBS 1X solution sterile?

Yes, PBS 1X is available in a sterile formulation, ensuring it is free from microbial contamination. This sterility is critical in laboratory settings where contamination can compromise experimental integrity. For example, using sterile PBS is essential when handling cultured cells or preparing immunological assays to prevent bacterial or fungal interference. Always check that the packaging is intact to maintain the sterility of the solution before use.

Can I use PBS for washing tissue samples?

Yes, PBS is commonly used for washing tissue samples before further processing or analysis. Its saline composition helps maintain osmotic balance, preventing tissue distortion during washing. This is particularly important when preparing samples for histological examination or when conducting assays that require intact tissue morphology. Many researchers utilize PBS to wash samples thoroughly, ensuring accurate results in subsequent steps.

Where can I buy Phosphate Buffered Saline, PBS 1X, Sterile, 500 mL?

You can buy Phosphate Buffered Saline, PBS 1X, Sterile, 500 mL at Ubuy in Dominican Republic. Ubuy offers a wide selection of laboratory supplies, ensuring availability and convenience for researchers looking for high-quality PBS solutions. Shopping through Ubuy gives you access to reliable products tailored for scientific applications.

¿Cómo comprar Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL en línea desde Ubuy?

Es fácil comprar Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.

¿Está Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL disponible para comprar en línea en Dominican Republic?

Sí, en Ubuy Dominican Republic, este producto está disponible para que lo compre a un precio razonable.. El Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.

¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL

1X Phosphate Buffered Saline (PBS) is a balanced, isotonic salt solution used for a variety of cell culture applications.

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Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL

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Nº de artículo: 51556604

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1X Phosphate Buffered Saline (PBS) is a balanced, isotonic salt solution used for a variety of cell culture applications.

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Sterile Solution

Ensures contamination-free experiments, enhancing the reliability and accuracy of your research outcomes with ready-to-use phosphate buffered saline.

Convenient Volume

500 mL quantity provides ample supply for multiple applications, reducing the need for frequent reorders and saving laboratory time and resources.

Optimal pH Stability

Maintains a stable pH environment, crucial for biological experiments, ensuring consistent performance across various applications in cell culture and immunology.

Detalles de producto

Manufactured in the USA in an ISO 9001 and 13485 certified facility
For research use only, not for human or animal clinical/therapeutic use
Each production lot undergoes stringent Good Manufacturing Practices and quality control tests
1X Phosphate Buffered Saline (PBS) is 0.22 micron filter-sterilized
Contains 137mM NaCl, 10mM NaH2PO4, 2.7mM KCl, 1.8mM KH2PO4, and has a pH of 7.4
Used for cell culture applications, such as washing cells, transporting cells or tissue, diluting cells for counting, and preparing reagents

Container Type	Bottle
Item Form	Liquid
Item Weight	8 ounces (226.8 grams)
Volume	500 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.95
Brand	eCon Lab Supply
Item Volume	500 ml
Package Information	Bottle

Who Should Buy?

Laboratory Research
Ideal for researchers conducting cell culture, protein purification, and immunoassays requiring a stable pH environment.
Diagnostic Testing
Useful for labs performing diagnostic tests, providing a sterile and buffered solution for sample preparation.
Biological Experiments
Suitable for biological experiments where maintaining osmotic balance and pH stability is critical for results.

Long-term Storage
Not suitable for long-term storage of cells or samples due to potential degradation and nutrient depletion.

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Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL

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Pregunta: ¿Cómo comprar Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL en línea desde Ubuy?

Respuesta: Es fácil comprar Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.
Pregunta: ¿Está Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL disponible para comprar en línea en Dominican Republic?

Respuesta: Sí, en Ubuy Dominican Republic, este producto está disponible para que lo compre a un precio razonable.. El Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.
Pregunta: ¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

Respuesta: El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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Limitaciones: Para los productos enviados al extranjero, ten en cuenta que cualquier garantía del fabricante puede no ser válida; las opciones de servicio del fabricante pueden no estar disponibles; los manuales del producto, las instrucciones y las advertencias de seguridad pueden no estar en los idiomas del país de destino; los productos (y los materiales que los acompañan) pueden no estar diseñados de acuerdo con las normas, especificaciones y requisitos de etiquetado del país de destino; y los productos pueden no ajustarse al voltaje del país de destino y a otras normas eléctricas (lo que requiere el uso de un adaptador o convertidor, si procede). El destinatario es responsable de asegurarse de que el producto puede ser importado legalmente al país de destino. Cuando hagas un pedido a Ubuy o a sus filiales, el destinatario es el importador registrado y debe cumplir todas las leyes y normativas del país de destino.
No todos los productos que aparecen en Ubuy están a la venta, ya que Ubuy es un motor de búsqueda a nivel mundial. Los productos están sujetos a las normas de exportación/comercio.

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características y beneficios

Contains a balanced, isotonic salt solution
Used to wash cells before dissociation
Transporting cells or tissue
Diluting cells for counting
Preparing numerous reagents
Manufactured under certified 14644 clean room conditions in USA lab

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Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL

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